Lipolytic activity of Malassezia spp. on inflamed seborrheic areas increases after exposure to beta-endorphin

Annotation. The opiate system of the skin modulates inflammation and regeneration, while metabolic products some bacteria fungi disrupt barrier cause inflammation. Therefore, study aimed to evaluate effect beta-endorphin (BE) on lipolytic activity (LA) Malassezia spp. isolated from both seborrheic areas healthy patients with dermatitis. included 42 moderate severe forms SD, positive growth spp., 17 medical workers without any diseases constituting control group. Two smears were taken SD patients: face, specifically inflamed affected by intact sternum. Samples transferred MLNA (37°C; 72 h), colonies suspended in Dixon broth, 500 nmol/L human BE was added (3 days; 32 °C). 1 ml suspension then applied yolk agar (7 35 precipitation zone considered a sign presence LA. following statistical research methods used: chi-square test, Mann-Whitney t-test, Student’s test. Half individuals did not show LA before after exposure. 69% samples obtained 95% had After stimulation, all In individuals, average value significant difference exposure (0.99 (0.99-1.00) vs 0.95 (0.92-0.97) p=0.358). intensity lipolysis significantly increased. areas, smaller than (0.06 p≤0.031; 0.22 p≤0.001). However, when comparing no found either or stimulation (0.91 (0.86-0.95) p=0.173). It established that increases which colonizes SD. Further using opioid receptor blockers for treatment dermatitis would be logical next step.